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Protein expression and purification The Expi293F cells transiently expressing CPT1B, TEX44 full-length (TEX44-FL), or the TEX44 C-terminal (TEX44-C, 385530aa) were harvested 60 h post-transfection and resuspended in lysis buffer (25 mM HEPES, pH 7.0, 150 mM NaCl, 2 mM EDTA) supplemented with a protease inhibitor cocktail (Roche)

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